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KMID : 0545119940040040338
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Journal of Microbiology and Biotechnology
1994 Volume.4 No. 4 p.338 ~ p.342
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Isolation, Identification, and Culture Conditions of the Strain Producing Eicosapentaenoic Acid
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Shin, Won Cheol
Kim, Chang Ho/Hong, Eock Kee
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Abstract
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The bacterium producing EPA was isolated from the intestines of the marine fishes. The strains were studied for their identification and their culture conditions. The selected strain was gram negative, rod (0.7×2.4μm in size) and motile with a single polar flagellum. This strain was identified as a Pseudomonas sp. on the basis of its morphological, cultural and physiological characteristics. The strain showed a maximum productivity of phospholipid at 20℃ after 48 hours of culture time with an initial pH of 7.0 in the PYM-glucose medium. Under these culture conditions, the production of phospholipid was about 0.3 ㎎/㎖ and 0.06 ㎎/㎎ dry cell weight.
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